It is generally recommended to store injections in the refrigerator, but if you accidentally missed doing so for one day, it should not cause any major harm. However, the effectiveness of the medication may be slightly reduced. The increased pain and discomfort you experienced could be due to other factors or unrelated to the temperature of the injection. If you continue to experience severe pain or any other concerning symptoms, it is important to consult with your healthcare provider for further evaluation and advice. They will be able to assess your specific situation and provide appropriate guidance based on your individual needs.