Yes, it is generally possible to determine the gender of your baby through ultrasound at 16 weeks. However, keep in mind that the accuracy of determining gender can vary depending on factors such as the position of the baby and the skill of the sonographer. It is important to note that some healthcare providers may prefer to wait until around 20 weeks for a more accurate determination. If you are eager to find out, talk to your healthcare provider about their policies and procedures regarding gender determination during ultrasounds.